2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Dutu za kawaida zinazotumika katika mkunjo wa urekebishaji wa usambazaji wa molekuli unaohusiana: insulini, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Ala na vifaa
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Kwa ujumla, uwiano wa amino asidi katika bidhaa za Sustar ni mkubwa kuliko ule wa bidhaa za Zinpro.
Sehemu ya 8 Athari za matumizi
Athari za vyanzo tofauti vya madini madogo kwenye utendaji wa uzalishaji na ubora wa mayai ya kuku wanaotaga katika kipindi cha mwisho cha kutaga
Mchakato wa Uzalishaji
Teknolojia ya chelation inayolengwa
Teknolojia ya uchanganyaji wa shear
Teknolojia ya kunyunyizia na kukausha kwa shinikizo
Teknolojia ya kuweka kwenye jokofu na kuondoa unyevunyevu
Teknolojia ya hali ya juu ya udhibiti wa mazingira
Kiambatisho A: Mbinu za Kuamua Usambazaji wa Masi ya Peptidi kwa Uzito wa Masi
Kupitishwa kwa kiwango: GB/T 22492-2008
1 Kanuni ya Mtihani:
Iliamuliwa kwa kutumia kromatografia ya uchujaji wa jeli yenye utendaji wa juu. Hiyo ni kusema, kwa kutumia kijazaji chenye vinyweleo kama awamu isiyosimama, kulingana na tofauti katika ukubwa wa molekuli wa vipengele vya sampuli kwa ajili ya utenganisho, iliyogunduliwa kwenye kifungo cha peptidi cha urefu wa wimbi la unyonyaji wa urujuanimno wa 220nm, kwa kutumia programu maalum ya usindikaji wa data kwa ajili ya kubaini usambazaji wa molekuli wa jamaa kwa kutumia kromatografia ya uchujaji wa jeli (yaani, programu ya GPC), kromatografia na data zao zilisindikwa, zikahesabiwa ili kupata ukubwa wa molekuli wa jamaa wa peptidi ya soya na safu ya usambazaji.
2. Vitendanishi
Maji ya majaribio yanapaswa kukidhi vipimo vya maji ya sekondari katika GB/T6682, matumizi ya vitendanishi, isipokuwa masharti maalum, ni safi kiuchambuzi.
2.1 Vitendanishi ni pamoja na asetonitrile (safi kikromatografiki), asidi trifluoroacetic (safi kikromatografiki),
2.2 Dutu za kawaida zinazotumika katika mkunjo wa urekebishaji wa usambazaji wa molekuli unaohusiana: insulini, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Ala na vifaa
3.1 Kromatografi ya Kioevu ya Utendaji wa Juu (HPLC): kituo cha kazi cha kromatografi au kiunganishi chenye kigunduzi cha UV na programu ya usindikaji wa data ya GPC.
3.2 Kifaa cha kuchuja na kuondoa gesi kwa awamu inayoweza kusogea.
3.3 Salio la kielektroniki: thamani iliyokadiriwa 0.000 1g.
Hatua 4 za uendeshaji
4.1 Hali za kromatografia na majaribio ya marekebisho ya mfumo (hali za marejeleo)
- 4.1.1 Safu wima ya kromatografi: TSKgelG2000swxl300 mm×7.8 mm (kipenyo cha ndani) au safu wima zingine za jeli za aina hiyo hiyo zenye utendaji sawa unaofaa kwa ajili ya kubaini protini na peptidi.
- 4.1.2 Awamu ya kuhama: Asetonitrile + maji + asidi trifluoroacetic = 20 + 80 + 0.1.
- 4.1.3 Urefu wa wimbi la kugundua: 220 nm.
- 4.1.4 Kiwango cha mtiririko: 0.5 mL/dakika.
- 4.1.5 Muda wa kugundua: dakika 30.
- 4.1.6 Kiasi cha sindano ya sampuli: 20μL.
- 4.1.7 Halijoto ya safu: halijoto ya chumba.
- 4.1.8 Ili kufanya mfumo wa kromatografia ukidhi mahitaji ya kugundua, ilielezwa kwamba chini ya hali za kromatografia zilizo hapo juu, ufanisi wa safu wima ya kromatografia ya jeli, yaani, idadi ya kinadharia ya sahani (N), haikuwa chini ya 10000 iliyohesabiwa kwa msingi wa vilele vya kiwango cha tripeptidi (Glycine-Glycine-Glycine).
- 4.2 Uzalishaji wa mikunjo sanifu ya uzito wa molekuli
- Suluhisho tofauti za kawaida za peptidi ya molekuli yenye mkusanyiko wa uzito wa 1 mg/mL zilitayarishwa kwa kulinganisha awamu inayotembea, zikachanganywa kwa uwiano fulani, na kisha kuchujwa kupitia utando wa awamu ya kikaboni wenye ukubwa wa vinyweleo vya 0.2 μm~0.5 μm na kuingizwa kwenye sampuli, na kisha kromatogramu za viwango zilipatikana. Mikunjo ya urekebishaji wa uzito wa molekuli na milinganyo yake ilipatikana kwa kuchora logarithm ya uzito wa molekuli unaohusiana dhidi ya muda wa uhifadhi au kwa urejeshaji wa mstari.
4.3 Sampuli ya matibabu
Pima kwa usahihi 10mg ya sampuli kwenye chupa ya ujazo ya 10mL, ongeza awamu kidogo inayoweza kuhamishika, ukitetemeka kwa ultrasound kwa dakika 10, ili sampuli iyeyuke kabisa na kuchanganywa, ikichanganywa na awamu inayoweza kuhamishika kwenye kipimo, na kisha kuchujwa kupitia utando wa awamu ya kikaboni wenye ukubwa wa vinyweleo vya 0.2μm ~ 0.5μm, na kichujio kilichambuliwa kulingana na hali ya kromatografi katika A.4.1.
- 5. Uhesabuji wa usambazaji wa molekuli ya jamaa
- Baada ya kuchanganua myeyusho wa sampuli ulioandaliwa katika 4.3 chini ya hali ya kromatografia ya 4.1, uzito wa molekuli wa sampuli na kiwango chake cha usambazaji kinaweza kupatikana kwa kubadilisha data ya kromatografia ya sampuli kwenye mkunjo wa urekebishaji 4.2 na programu ya usindikaji wa data ya GPC. Usambazaji wa wingi wa molekuli wa peptidi tofauti unaweza kuhesabiwa kwa njia ya urekebishaji wa eneo la kilele, kulingana na fomula: X=A/A jumla×100
- Katika fomula: X - Sehemu ya uzito wa peptidi ya molekuli inayohusiana katika peptidi yote katika sampuli, %;
- A - Eneo la kilele cha peptidi ya molekuli inayohusiana;
- Jumla A - jumla ya maeneo ya kilele cha kila peptidi ya molekuli inayohusiana, iliyohesabiwa hadi sehemu moja ya desimali.
- 6 Kurudia
- Tofauti kamili kati ya maamuzi mawili huru yaliyopatikana chini ya masharti ya kurudiwa hayatazidi 15% ya wastani wa hesabu wa maamuzi hayo mawili.
- Kiambatisho B: Mbinu za Kubaini Asidi za Amino Huru
- Kupitishwa kwa kiwango: Q/320205 KAVN05-2016
- 1.2 Vitendanishi na vifaa
- Asidi ya asetiki ya barafu: safi kiuchambuzi
- Asidi ya perkloriki: 0.0500 mol/L
- Kiashiria: Kiashiria cha violet ya fuwele ya 0.1% (asidi asetiki ya barafu)
- 2. Uamuzi wa asidi amino huru
Sampuli zilikaushwa kwa joto la 80°C kwa saa 1.
Weka sampuli kwenye chombo kikavu ili ipoe kiasili hadi joto la kawaida au ipoe hadi joto linalofaa kutumika.Pima takriban 0.1 g ya sampuli (sahihi hadi 0.001 g) kwenye chupa kavu ya umbo la koni ya mL 250.Endelea haraka hadi hatua inayofuata ili kuepuka sampuli kunyonya unyevunyevu wa mazingiraOngeza mililita 25 za asidi asetiki ya barafu na uchanganye vizuri kwa si zaidi ya dakika 5.Ongeza matone 2 ya kiashiria cha violet ya fuwelePunguza kwa kutumia suluhisho la kawaida la titration la 0.0500 mol / L (±0.001) la asidi ya perkloriki hadi suluhisho libadilike kutoka zambarau hadi ncha ya mwisho.
Andika kiasi cha suluhisho la kawaida linalotumiwa.
- Fanya jaribio tupu kwa wakati mmoja.
- 3. Hesabu na matokeo
- Kiwango cha amino asidi huru X katika kitendanishi huonyeshwa kama sehemu ya wingi (%) na huhesabiwa kulingana na fomula: X = C × (V1-V0) × 0.1445/M × 100%, katika fomula ya tne:
- C - Mkusanyiko wa myeyusho wa kawaida wa asidi ya pekloriki katika moles kwa lita (mol/L)
- V1 - Kiasi kinachotumika kwa ajili ya ugawaji wa sampuli kwa kutumia myeyusho wa kawaida wa asidi ya pekloriki, katika mililita (mL).
- Vo - Kiasi kinachotumika kwa titration tupu na suluhisho la kawaida la asidi ya perchloric, katika mililita (mL);
M - Uzito wa sampuli, katika gramu (g).
| 0.1445: Uzito wa wastani wa amino asidi sawa na mL 1.00 ya myeyusho wa kawaida wa asidi ya pekloriki [c (HClO4) = 1.000 mol/L]. | 4.2.3 Mmumunyo wa kiwango cha titration wa seriamu salfeti: mkusanyiko c [Ce (SO4) 2] = 0.1 mol/L, iliyoandaliwa kulingana na GB/T601. | |
| Kupitishwa kwa viwango: Q/70920556 71-2024 | 1. Kanuni ya uamuzi (Fe kama mfano) | Misombo ya chuma ya asidi amino ina umumunyifu mdogo sana katika ethanoli isiyo na maji na ioni za metali huru huyeyuka katika ethanoli isiyo na maji, tofauti ya umumunyifu kati ya hizo mbili katika ethanoli isiyo na maji ilitumika kubaini kiwango cha chelation cha misombo ya chuma ya asidi amino. |
| Katika fomula: V1 - ujazo wa suluhisho la kawaida la cerium sulfate linalotumiwa kwa ajili ya kuongeza titration ya suluhisho la majaribio, mL; | Ethanoli isiyo na maji; iliyobaki ni sawa na kifungu cha 4.5.2 katika GB/T 27983-2011. | 3. Hatua za uchambuzi |
| Fanya majaribio mawili sambamba. Pima 0.1g ya sampuli iliyokaushwa kwa 103±2℃ kwa saa 1, sahihi kwa 0.0001g, ongeza 100mL ya ethanol isiyo na maji ili kuyeyusha, chuja, chuja mabaki yaliyooshwa na 100mL ya ethanol isiyo na maji kwa angalau mara tatu, kisha uhamishe mabaki kwenye chupa ya umbo la koni ya 250mL, ongeza 10mL ya myeyusho wa asidi ya sulfuriki kulingana na kifungu cha 4.5.3 katika GB/T27983-2011, na kisha fanya hatua zifuatazo kulingana na kifungu cha 4.5.3 "Pasha ili kuyeyusha kisha uache ipoe" katika GB/T27983-2011. Fanya jaribio tupu kwa wakati mmoja. | 4. Uamuzi wa jumla ya kiwango cha chuma | 4.1 Kanuni ya uamuzi ni sawa na kifungu cha 4.4.1 katika GB/T 21996-2008. |
4.2. Vitendanishi na Suluhisho
| 4.2.1 Asidi Mchanganyiko: Ongeza mililita 150 za asidi ya sulfuriki na mililita 150 za asidi ya fosforasi kwenye mililita 700 za maji na uchanganye vizuri. | 4.2.2 Myeyusho wa kiashiria cha sodiamu difenilamini salfonate: 5g/L, ulioandaliwa kulingana na GB/T603. | 4.2.3 Mmumunyo wa kiwango cha titration wa seriamu salfeti: mkusanyiko c [Ce (SO4) 2] = 0.1 mol/L, iliyoandaliwa kulingana na GB/T601. | |
| 4.3 Hatua za uchambuzi | Fanya majaribio mawili kwa wakati mmoja. Pima 0.1g ya sampuli, sahihi kwa 020001g, weka kwenye chupa ya umbo la koni ya 250mL, ongeza 10mL ya asidi mchanganyiko, baada ya kuyeyuka, ongeza 30ml ya maji na matone 4 ya mchanganyiko wa kiashiria cha sodiamu dianiline sulfonate, kisha fanya hatua zifuatazo kulingana na kifungu cha 4.4.2 katika GB/T21996-2008. Fanya jaribio tupu kwa wakati mmoja. | 4.4 Uwakilishi wa matokeo | Jumla ya kiwango cha chuma X1 cha amino asidi tata za chuma kwa upande wa sehemu ya uzito wa chuma, thamani iliyoonyeshwa kwa %, ilihesabiwa kulingana na fomula (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - suluhisho la kawaida la seriamu salfeti linalotumiwa kwa ajili ya kuongeza titration ya suluhisho tupu, mL; | V0 - suluhisho la kawaida la seriamu salfeti linalotumiwa kwa ajili ya kuongeza titration ya suluhisho tupu, mL; | C - Mkusanyiko halisi wa suluhisho la kawaida la cerium sulfate, mol/L5. Uhesabuji wa kiwango cha chuma katika chelatesKiwango cha chuma X2 katika chelate kwa upande wa sehemu ya uzito wa chuma, thamani iliyoonyeshwa kwa %, ilihesabiwa kulingana na fomula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Katika fomula: V1 - ujazo wa suluhisho la kawaida la cerium sulfate linalotumiwa kwa ajili ya kuongeza titration ya suluhisho la majaribio, mL; | V2 - suluhisho la kawaida la seriamu salfeti linalotumiwa kwa ajili ya kuongeza titration ya suluhisho tupu, mL;nom1 - Uzito wa sampuli, g. Chukua wastani wa hesabu wa matokeo ya uamuzi sambamba kama matokeo ya uamuzi, na tofauti kamili ya matokeo ya uamuzi sambamba si zaidi ya 0.3%. | 0.05585 - uzito wa chuma chenye feri ulioonyeshwa katika gramu sawa na mL 1.00 ya myeyusho wa kawaida wa sulfate ya seriamu C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1 - Uzito wa sampuli, g. Chukua wastani wa hesabu wa matokeo ya uamuzi sambamba kama matokeo ya uamuzi, na tofauti kamili ya matokeo ya uamuzi sambamba si zaidi ya 0.3%. | 6. Hesabu ya kiwango cha chelationKiwango cha Chelation X3, thamani iliyoonyeshwa kwa %, X3 = X2/X1 × 100Kiambatisho C: Mbinu za Kuamua Kiwango cha Chelation cha Zinpro |
Kupitishwa kwa kiwango: Q/320205 KAVNO7-2016
1. Vitendanishi na vifaa
a) Asidi ya asetiki ya barafu: safi kiuchambuzi; b) Asidi ya perkloriki: 0.0500mol/L; c) Kiashiria: Kiashiria cha violet ya fuwele 0.1% (asidi ya asetiki ya barafu)
2. Uamuzi wa asidi amino huru
2.1 Sampuli zilikaushwa kwa joto la 80°C kwa saa 1.
2.2 Weka sampuli kwenye chombo kikavu ili ipoe kiasili hadi joto la kawaida au ipoe hadi joto linalofaa kutumika.
2.3 Pima takriban 0.1 g ya sampuli (sahihi hadi 0.001 g) kwenye chupa kavu ya umbo la koni ya mL 250
2.4 Endelea haraka hadi hatua inayofuata ili kuepuka sampuli kunyonya unyevunyevu wa mazingira.
2.5 Ongeza 25mL ya asidi asetiki ya barafu na uchanganye vizuri kwa si zaidi ya dakika 5.
2.6 Ongeza matone 2 ya kiashiria cha violet ya fuwele.
2.7 Titrate na 0.0500mol/L (±0.001) suluhisho la kawaida la titration la asidi ya pekloriki hadi myeyusho ubadilike kutoka zambarau hadi kijani kwa sekunde 15 bila kubadilisha rangi kama sehemu ya mwisho.
2.8 Andika kiasi cha myeyusho wa kawaida unaotumiwa.
2.9 Fanya jaribio lisilo na kitu kwa wakati mmoja.
- 3. Hesabu na matokeo
- Kikatalani
- Physicochemical parameters
V1 - Kiasi kinachotumika kwa ajili ya ugawaji wa sampuli kwa kutumia myeyusho wa kawaida wa asidi ya pekloriki, katika mililita (mL).
Vo - Kiasi kinachotumika kwa titration tupu na suluhisho la kawaida la asidi ya perchloric, katika mililita (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Anwani: Nambari 147 Barabara ya Qingpu, Mji wa Shouan, Kaunti ya Pujiang, Jiji la Chengdu, Mkoa wa Sichuan, Uchina
Simu: 86-18880477902
Bidhaa
Madini ya kufuatilia yasiyo ya kikaboni
- Madini ya kikaboni
- Kiswahili
- Huduma maalum
- Viungo vya haraka
Wasifu wa Kampuni
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Kigujarati | Bonyeza kwa uchunguzi | © Hakimiliki - 2010-2025: Haki Zote Zimehifadhiwa. | Ramani ya tovuti UTAFITI WA JUU Simu |
| Simu | 86-18880477902 | Kijava | Barua pepe |
| 8618880477902 | Kichina | Kifaransa | |
| Bird | Kichina | Kifaransa | Kijerumani Kihispania |
| Aquatic animals | Kijapani | Kikorea | Kiarabu Kigiriki |
| Kituruki | Kiitaliano | ||
| Ruminant animal g/head day | January 0.75 | Kiindonesia Kiafrikana Kiswidi |
Kipolandi
- Kibasque
- Kikatalani
- Physicochemical parameters
Kihindi
Kilao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Kishona
Kibulgaria
- Kisebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Kikroeshia
Kiholanzi
| Application object | Kiurdu Kivietnam | Content in full-value feed (mg/kg) | Efficacy |
| Kigujarati | Kihaiti | Kihausa | Kinyarwanda Kihmong Kihungaria |
| Piglets and fattening pigs | Igbo | Kijava | Kikannada Kikhmer Kikurdi |
| Kyrgyz | Kilatini | ||
| Bird | 300～400 | 45～60 | Kimasedonia Kimalei Kimalayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Kinorwe
- Kipashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Kiserbia
Kisesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Kishona
Kisindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Kiswahili
Tajik
Kitamil
Kitelugu
Kithai
| Application object | Kiurdu Kivietnam | Content in full-value feed (mg/kg) | Efficacy |
| Kiyidi | Kiyoruba | Kizulu | Kinyarwanda Kioriya Waturuki |
| Uyghur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
